Antiviral activities of Radix isatidis polysaccharide against pseudorabies virus in swine testicle cells

Yanyan Qiao,Zewen Chen,Xuebing Wang,Fang Liu,Tong Chen,Chao Tong

doi:10.1186/s12906-020-2838-4

Yanyan Qiao, Zewen Chen + Show 4 more

Open Access

https://doi.org/10.1186/s12906-020-2838-4

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Abstract

BackgroundRadix isatidis has been used in China and other Asian countries for its antiviral and anti-inflammatory effects for thousands of years. However, the antiviral effect of Radix isatidis polysaccharide against pseudorabies virus (PRV) is still unknown.MethodsThe polysaccharide were isolated from extract of the roots of Radix isatidis. MTT assays were used to determine the preventive effect, inhibitory effect and antiviral effect of Radix isatidis polysaccharide on PRV in vitro.ResultsThis study found that different concentrations of polysaccharides from this plant can inhibit PRV replication by 14.674–30.840%, prevent infection at rates of 6.668–14.923%, and kill this virus at rates of 32.214–67.422%.ConclusionThese results broaden the understanding of this traditional Chinese herb and provide a theoretical basis for further research. Moreover, Radix isatidis polysaccharide could be used for antiviral therapy.

Highlights

Radix isatidis has been used in China and other Asian countries for its antiviral and anti-inflammatory effects for thousands of years
Few studies have focused on this point and in this study, we explored the antiviral effect of Radix isatidis polysaccharide using swine testicle cells and investigated its protective effect against pseudorabies virus (PRV) infection
Toxicity of Radix isatidis polysaccharide toward swine testicle cells The cytotoxic effect of Radix isatidis polysaccharide extracts on porcine testicle cells was determined by the MTT method after a 68-h co-incubation

Summary

Introduction

Radix isatidis has been used in China and other Asian countries for its antiviral and anti-inflammatory effects for thousands of years. The antiviral effect of Radix isatidis polysaccharide against pseudorabies virus (PRV) is still unknown. MTT assays were used to determine the preventive effect, inhibitory effect and antiviral effect of Radix isatidis polysaccharide on PRV in vitro. It can lead huge economic losses to the global swine industry as PRV epidemics can lead to an average loss of $6/cwt [2, 3]. After young pigs or other susceptible species are infected, PRV is often lethal and can lead to central nervous system disorders [5]. It can cause huge economic losses to the swine industry in many countries [6]. Despite the fact that humans are not typically susceptible to infection, one case of PRV resulting in infectious endophthalmitis was reported [7]. Since the first antiviral drug was approved in 1963, many

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