ANTIVIRAL ACTION OF AMINOPROPANOL-2 DERIVATIVES ON THE EXPERIMENTAL MODELS OF HEPATITIS C VIRUS

Abstract

Aim. The purpose of the work is to investigate the antiviral action of new aminopropanol-2 compounds against bovine viral diarrhea virus (BVDV) and on the experimental hepatitis C virus (HCV) production model. Methods. Antiviral activity was determined in 7 aminopropanol-2 derivatives, among which there were: norbornyl-containing substance (compound № 51), a substance with a cyclic substituent in the alkoxy group (compound № 48), and the substances with alicyclic substituents in the alkoxy group (compounds №№ 46, 47, 49, 50 and 52). The evaluation of antiviral effect of the studied substances was conducted in vitro according to their chemo-therapeutic indices (HTI) and polymerase chain reaction (PCR) in the experimental HCV model. Results. It was found that compounds №№ 50 and 52 had high HTI and inhibited the reproduction of BVDV by 2 lg ID50 at concentrations of 3,125 and 6.25 μg/ml, respectively, that characterizes these substances as the effective BVDV reproduction inhibitors. The antiviral activity of compound № 50 was confirmed by PCR data on the experimental hepatitis C virus (HCV) production model. There were also shown the dose-dependent effect of this substance on the reproduction of hepatitis C virus. Conclusions. Among the investigated aminopropanol-2 derivatives there were identified a compound with pronounced antiviral properties. This is 1-tret-butoxy-3-(2,2,6,6-tetramethyl-4-hydroxypiperidino)-2-propanol (substance №50) which belongs to the group of substances with alicyclic substituents in the alkoxi group. The obtained results can be used for a purposeful synthesis of active molecules with given properties and will be useful in studying the relationships of structure-activity.