Anti-Vascular Inflammatory Effect of Ethanol Extract from Securinega suffruticosa in Human Umbilical Vein Endothelial Cells.

Byung Hyuk Han,Chang Seob Seo,Chun Ho Song,Jung Joo Yoon,Ho Sub Lee,Dae Gill Kang,Hye Yoom Kim,Yun Jung Lee

doi:10.3390/nu12113448

Byung Hyuk Han, Chang Seob Seo + Show 6 more

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https://doi.org/10.3390/nu12113448

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Abstract

Securiniga suffruticosa is known as a drug that has the effect of improving the blood circulation and relaxing muscles and tendons, thereby protects and strengthen kidney and spleen. Therefore, in this study, treatment of Securiniga suffruticosa showed protective effect of inhibiting the vascular inflammation in human umbilical vein endothelial cells (HUVECs) by inducing nitric oxide (NO) production and endothelial nitric oxide synthase (eNOS) coupling pathway. In this study, Securiniga suffruticosa suppressed TNF-α (Tumor necrosis factor–α) induced protein and mRNA levels of cell adhesion molecules such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and Interleukin-6 (IL-6). Pretreatment of HUVEC with Securiniga suffruticosa decreased the adhesion of HL-60 cells to Ox-LDL (Oxidized Low-Density-Lipoprotein)-induced HUVEC. Moreover, Securiniga suffruticosa inhibited TNF-α induced intracellular reactive oxygen species (ROS) production. Securiniga suffruticosa also inhibited phosphorylation of IκB-α in cytoplasm and translocation of NF-κB (Nuclear factor-kappa B) p65 to the nucleus. Securiniga suffruticosa increased NO production, as well increased the phosphorylation of eNOS and Akt (protein kinase B) which are related with NO production. In addition, Securiniga suffruticosa increased the protein expression of GTPCH (Guanosine triphosphate cyclohydrolase Ⅰ) and the production of BH4 in HUVEC which are related with eNOS coupling pathway. In conclusion, Securiniga suffruticosa has a protective effect against vascular inflammation and can be a potential therapeutic agent for early atherosclerosis.

Highlights

Vascular inflammation is a condition which plays a key process in the disorder of the endothelium.Endothelium is activated in the inflammatory site where a number of leukocytes adhere to the vascular endothelial layer and infiltrate into the vessel wall and differentiate into macrophages
The expressions of phosphorylated endothelial nitric oxide synthase (eNOS) and Akt increased in dose-dependent manner, compared to the total forms of the respective proteins. These results suggest that Securinega suffruticosa stimulates p-eNOS and p-Akt expression in human umbilical vein endothelial cells (HUVECs)
This study found whether Securiniga suffruticosa suppressed monocytes adhesion to TNF-α-stimulated HUVEC by suppressing expression of cell adhesion molecules and pro-inflammatory cytokines such as vascular cell adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1) and IL-6

Summary

Introduction

Vascular inflammation is a condition which plays a key process in the disorder of the endothelium. Endothelium is activated in the inflammatory site where a number of leukocytes adhere to the vascular endothelial layer and infiltrate into the vessel wall and differentiate into macrophages. This is very important in the early steps of development of endothelial dysfunction and tissue damage [1]. Vascular inflammation and macrophage differentiation are regarded as very important step. The process of inflammatory cytokine is produced by various cells such as macrophages, lymphocytes, neutrophils and mast cells

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