Antityrosinase and antioxidant activity of asparagus and its inhibition on B16F10 melanoma cells before and after hydrothermal treatment

Abstract

In this study, we compared the effects of asparagus polyphenol extract (ASE) and asparagus polyphenol extract after hydrothermal treatments (ASEH) on antityrosinase and antioxidant activity, in addition to murine melanoma B16F10 cells. The intracellular experiments of four fractions, including cell morphology, cell viability, melanin content and intracellular tyrosinase activity were evaluated after 24, 48, and 72 h of treatment. The results indicated that the enhancement of gallic acid, coumaric acid, and hydroxybenzoic acid contents could be major reasons for the increased antityrosinase ability. Hydrothermal treatments could significantly increase the antityrosinase and antioxidant activity of asparagus polyphenol extracts. Meanwhile, ASEH (25–200 μg/mL) decreased the melanin content in B16F10 cells from 56.18% to 21.99% in a concentration-dependent manner. Furthermore, the intracellular tyrosinase activity of B16F10 cells was 39.78% at an ASEH concentration of 200 μg/mL. These results demonstrated that hydrothermal treatment was an efficient method to enhance the antityrosinase and antioxidant activity of asparagus polyphenols.