Antitumour Activities of Selected Pure Compounds Identified from the Serum of Crocodylus porosus, Malayopython reticulatus, Varanus salvator and Cuora kamaroma amboinensis

Shareni Jeyamogan,Ruqaiyyah Siddiqui,Naveed Ahmed Khan

doi:10.31557/apjcp.2021.22.s1.97

Abstract

Here we determined antitumour effects of purified compounds such as Valdecoxib, Rofecoxib, L-Methionine and Artocarpin against cancer cell lines. Using purified compounds, assays were performed to determine their effects against cancer cell lines using growth inhibition assays, cytotoxicity assays, and cell survival assays against HeLa, PC3 and MCF7 cells. The results showed that the selected small molecules L-Methionine, Rofecoxib, and Artocarpin suppressed the growth of more than 90% PC3 cells at 40µM. Similarly, Valdecoxib alone and in combination with other molecules exhibited potent growth inhibition and cytotoxicity against cancer cells tested. Peptide from the serum of M. reticulatus, demonstrated selective cytotoxicity against cancer cells without inhibiting the growth of normal cells. These findings are significant and provide a basis for the rational development of therapeutic anticancer agents, however intensive research is needed to determine in vivo effects of the identified molecules together with their mode of action to realize these expectations.<br />.

Highlights

In spite of the advances in treatment modalities and supportive care, significant rise in cancer morbidity and mortality highlights the need for the discovery of new effective anticancer agents (Bray et al, 2018; Ferlay et al, 2015; Parkin et al, 2001; Siegel et al, 2020)
For the identification of potential Anticancer Peptides candidates, the Machine Learning Based Prediction of Anticancer Peptides (MLACP) methodology was used that employs the SVM (SVMACP) and Random Forest (RFACP) method to calculate the probability of a peptide in being a potential anticancer peptide using a combinations of features from the peptide sequence, amino acid composition (AAC), dipeptide composition (DPC), Atomic composition (ATC) and physicochemical properties (PCP)
The results revealed that the combination of 40μM L-Methionine, 40μM Rofecoxib and 40μM Artocarpin, significantly suppressed the growth of more than 90% PC3 cells (P

Summary

Introduction

In spite of the advances in treatment modalities and supportive care, significant rise in cancer morbidity and mortality highlights the need for the discovery of new effective anticancer agents (Bray et al, 2018; Ferlay et al, 2015; Parkin et al, 2001; Siegel et al, 2020). The search for potential anticancer molecules from the animal Kingdom largely remains an untapped source Reptiles such as crocodiles, snakes and water monitor lizards, thrive in contaminated and polluted environments exposing them to toxic and carcinogenic heavy metal elements such as selenium, cadmium, mercury, arsenic, chromium, nickel, cobalt and zinc (Lehner et al, 2013; Tellez and Merchant, 2015; Xu et al, 2006). These findings led to the identification of numerous molecules with anticancer potential

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