Abstract
Green synthesized silver nanoparticles (AgNPs) have gained considerable interest because of their potential applicability in numerous fields, including biomedicine. This study proposed a green, rapid, and effective synthesis of AgNPs using Punica granatum extracts and sunlight for effective breast cancer treatment. Phytochemical analysis of Punica granatum extracts confirmed the existence of phyto-compounds, which are responsible for coating and stabilizing the AgNPs. The optimization study suggested that rapid synthesis of AgNPs can be accomplished using sunlight. Fourier transform infrared spectroscopy analysis confirmed bio-compounds deposition in P. granatum extracts on the AgNPs. The results showed that the biosynthesized AgNPs were highly stable at −27 mV and spherical in sizes from 13 to 37 nm. The anticancer activity evaluation results demonstrated that the AgNPs using P. granatum under sunlight possess significant antiproliferative and apoptosis-inducing activities in breast cancer cells. The apoptotic properties of AgNPs using P. granatum leaves (AgNPs-PL) were assessed by morphological observations, DAPI staining, DNA fragmentation assay, and flow cytometry. The results show the potential therapeutic value of the biosynthesized AgNPs for further development as anticancer drugs.
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