Antitumor effect of recombinant human endostatin combined with cisplatin on rats with transplanted Lewis lung cancer.

Abstract

To observe the antitumor effect and mechanism of recombinant human endostatin (Endostar) injection in tumor combined with intraperitoneal injection of cisplatin on subcutaneous transplanted Lewis lung cancer in rats. A total of 30 C57 rats were selected, and the monoplast suspension of Lewis lung cancer was injected into the left axilla to prepare the subcutaneous transplanted tumor models in the axilla of right upper limb. The models were randomly divided into Groups A, B, and C. Medication was conducted when the tumor grew to 400mm(3). Group A was the control group without any interventional treatment. Group B was injected with Endostar 5mgkg(-1)d(-1) for 10d. Group C was given the injection of Endostar 5mgkg(-1)d(-1) combined with intraperitoneal injection of cisplatin 5mgkg(-1)d(-1) for 10d. All the rats in three groups were executed the day after the 10 d medication and the tumor was taken off for measurement of volume and mass changes and calculation of antitumor rate, after which the vascular endothelial growth factor (VEGF) concentration in rats' plasma was determined by ELISA. The tumor tissues were cut for the preparation of conventional biopsies. After hematoxylin-eosin staining, the pathologic histology was examined to observe the structures of tumor tissues, VEGF score and microvessel density (MVD) in each group. The volume and mass of tumor in Groups B and C were significantly lower than Group A (P<0.05) while the tumor volume and mass in Group C were significantly lower than Group B (P<0.05). The antitumor rate in Group C was significantly higher than Group B (P<0.05), but the tumor VEGF score, MVD and plasma VEGF level in Group C were significantly lower than Groups A and B (P<0.05). In Group B, the tumor VEGF score, MVD and plasma VEGF level were significantly lower than Group A (P<0.05). The microscopic image of Group C showed that its number of active tumor cells and the blood capillary around tumor was significantly smaller than that of Groups A and B, and meanwhile atrophy and liquefactive necrosis were seen in local tumor. Endostar injection combined with intraperitoneal injection of cisplatin is effective in reducing tumor VEGF score and MVD of transplanted tumor tissues in rats with Lewis lung cancer to obstruct the nutrient supply of tumor cells and kill tumor cells, so that the inhibition of tumor cell proliferation and metastasis can be achieved with a remarkable effect.