Antitumor effect of dendritic cell-based cancer vaccine in combination with chemotherapy in patients with advanced colorectal cancer.

Abstract

e13103 Background: Tumor-specific cytotoxic T lymphocytes (CTLs) can be activated in vivo by dendritic cell (DC)- based vaccination. However, clinical responses to the immunotherapy with DC vaccination have only been observed in a minority of patients with solid cancer. Combination with other treatment modalities such as chemotherapy may overcome immunoresistance of cancer cells. In the current study, the clinical efficacy of the DC vaccine pulsed with the peptide derived from colorectal cancer-associated antigens in combination with chemotherapy has been evaluated in patients with advanced, inoperable colorectal cancer. Methods: Thirty-two patients with advanced, inoperable colorectal cancer refractory to standard treatment were entered into the study. DCs that were generated from CD14+ monocytes from leukapheresis by 6-day cultivation with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4, were matured by OK-432, a streptococcal agent, and were pulsed with the pancreatic c...