Anti-tumor effect of cisplatin and adriamycin combination on proliferation of tongue squamous carcinoma cell line Tca8113

Abstract

Objective To study the anti-tumor effect of cisplatin and adriamycin combination on the growth, cell cycle and apoptosis of tongue squamous cancer cell line Tca8113.Methods Different concentrations of cisplatin and/or adriamycin were added to Tca8113 cells.Cells proliferation was detected by MTT assay, and cell cycle and apoptosis detected by flow cytometry.Results Cisplatin(r=0.538, P<0.01) and adriamycin (r=0.642, P<0.01)inhibited cell growth in a dose- and time-dependent manner.Addition of 1.25 μmol/L adriamycin alone for 24 hours did not show any inhibition on the growth of Tea8113.With cisplatin (2～10 mg/L) alone for 24 hours, only 10 mg/L cisplatin inhibited the growth of TcaSll3 cells significantly (P<0.01).Combined with 1.25 μmol/L adriamycin, 24-hour treatment of cisplastin inhibited the cell growth at the levels of 4, 6, 8 and 10 mg/L which was significantly different as compared to the same level of cisplatin alone (all P<0.001).1.25 μmol/L adriamycin, 6 mg/L cisplatin and adriamycin 1.25 μmol/L + cisplatin 6 mg/L were all shown to significantly change G_0-G_1 phase (50.00±0.32%, 43.53±2.026%, 50.77± 3.83%) and S phase cell percentage (36.5±1.05%,40.8±2.52%, 30.87±2.65%) (all P<0.01), but the effect was more evident with combination of cisplatin and adriarnycin (P<0.05).While no obvious effect on apoptosis was found with these drags, combined use led to higher percentage of necrotizing cells.Conclusion Cisplatin and adriamycin combination can markedly inhibit the celia line Tca8113 differentiation and proliferation, and enhance cytotoxic effect of these two drugs. Key words: Tongue carcinoma; Cisplatin; Adriamycin; Growth inhibition; Cell cycle