Antitumor effect of a hydroalcoholic extract of Uncaria tomentosa in a murine C57BL/6- B16 melanoma model

Abstract

Event Abstract Back to Event Antitumor effect of a hydroalcoholic extract of Uncaria tomentosa in a murine C57BL/6- B16 melanoma model Yubell P. Alvarez1*, Ivan A. Lozada1, Jose L. Aguilar1, Silvia Dávila1, Claudia Ocampo1, Claudio Acuña2, Abraham Vaisberg3, Tindara Ysmodes1 and Cesar Nunez4 1 Universidad Peruana Cayetano Heredia, Lima, Peru 2 Universidad de Santiago de Chile, Peru 3 Universidad Peruana Cayetano Heredia, Peru 4 Empresa de Investigación y Desarrollo en Cáncer, EMINDES SAC, Peru Uncaria tomentosa extracts have shown immunomodulatory, antiproliferative and antitumor activity. However, little is known about its effects in immunocompetent cells or Th1/Th2/Th17 cytokines production. Objective: To evaluate antitumor effect of an hydroalcoholic extract of Uncaria tomentosa (Cat's Claw) containing 5.03% pentacyclic oxindole alkaloids (UG-POA) in a murine model for melanoma C57BL/6-B16 by measuring lymphocytes, Th1/Th2/Th17 cytokines and tumor weight and volume. Methodology: Treatment with UG-POA extracts (50,500,100 mg/kg) or its dilution vehicle was given orally 7 days before and 21 days after tumor induction with 1x105 B16. Animals were sacrificed on day 22. Weight and tumor volume were assessed. Peripheral blood mononuclear cells (PBMC) were obtained, and analyzed by flow cytometry. Culture supernatants were collected to measure the production of Th1/th2/th17 using Cytometric Bead Array (CBA) . +Results: We found a dose-dependent increase of CD3 + CD4 + LT, being statically significant at 50 and 1000 mg/kg compared with positive control group. However, CD4 + CD44 + LT showed a significant dose-dependent decrease compared with positive control group. CD3 + CD8 + LT population showed no differences in relation with positive control, except for a decrease in 50 mg/kg treated group. When evaluating Th1/th2/th17, we only found an increase in IL-6 in a dose dependent fashion, being significant at 1000 mg/kg. Tumor weight and volumes showed no significant differences. Conclusions: It´s likely that UG-POA exhibits an anti-tumor effect modulating the increase and activation of LT CD4+ and IL6, an important cytokine in the host's immune and metabolic responses to cancer. Keywords: Uncaria tomentosa, Melanoma, Mice, Cytokines, B16 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Alvarez YP, Lozada IA, Aguilar JL, Dávila S, Ocampo C, Acuña C, Vaisberg A, Ysmodes T and Nunez C (2013). Antitumor effect of a hydroalcoholic extract of Uncaria tomentosa in a murine C57BL/6- B16 melanoma model. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00143 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 09 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Yubell P Alvarez, Universidad Peruana Cayetano Heredia, Lima, Lima, Peru, yubi_99@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Yubell P Alvarez Ivan A Lozada Jose L Aguilar Silvia Dávila Claudia Ocampo Claudio Acuña Abraham Vaisberg Tindara Ysmodes Cesar Nunez Google Yubell P Alvarez Ivan A Lozada Jose L Aguilar Silvia Dávila Claudia Ocampo Claudio Acuña Abraham Vaisberg Tindara Ysmodes Cesar Nunez Google Scholar Yubell P Alvarez Ivan A Lozada Jose L Aguilar Silvia Dávila Claudia Ocampo Claudio Acuña Abraham Vaisberg Tindara Ysmodes Cesar Nunez PubMed Yubell P Alvarez Ivan A Lozada Jose L Aguilar Silvia Dávila Claudia Ocampo Claudio Acuña Abraham Vaisberg Tindara Ysmodes Cesar Nunez Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.