Antitumor and antioxidant activity of Aloe vera leaf gel extract

Abstract

The purpose of the study was to determine the In-vitro antitumor andantioxidant activity of Aloe vera gel extract by evaluating the cytotoxic effect of extract and Zoladex (goserelin) on breast cancer cell line (MCF- 7) and prostate cancer cell line (PC-3), in addition to measure the antioxidant activity to find out this kind of anticancer drug, which is a cheap, safe, less toxic, and more active drug contrasted to chemotherapy drug. The cytotoxic effect evaluated by cytotoxicity assay (MTT assay), while the activity of the Aloe vera gel as antioxidant was tested using a scavenging test of free radical (2,2-Diphenyl-1-picrylhydrazyl) (DPPH), ferric reducing antioxidant potential (FRAP) and (Oxygen Radical Absorbance Capacity)(ORAC). Treatment of the extract against breast cancer cell line (MCF-7) and prostate cancer cell line (PC-3), in all concentrations, indicated a decrease in cell viability percentage with the concentration increasing. Aloe vera extract showed its best activity in the concentration of 400 μg/ml. The same results observed with Zoladex treatment for breast cancer cell line (MCF-7) and prostate cancer cell line (PC-3) which represented a decrease in cell viability percentage with the concentration increasing. Aloe vera gel extract was more than to the activity of Zoladex drug and revealed that Aloe vera extract exhibits good cytotoxic activity. The antioxidant properties of the Aloe vera gel extract was effective. Antioxidant activities of Aloe vera gel extract were estimated using different concentrations starting with 200 μg/ml, 100 μg/ml, 50 μg/ml, 25 μg/ml and, 12.5 μg/ml. Aloe vera gel extract have the maximum scavenging activity at 200 μg/ml.