Antitumor Activity of Some Polysaccharides Isolated from an Edible Mushroom,Ningyotake, the Fruiting Body and the Cultured Mycelium ofPolyporous confluens

Abstract

Some polysaccharides extracted from ningyotake, the fruiting body of Polyporous confluens, with hot water (100°C, FI), 1% ammonium oxalate solution (100°C, FII), and 5% sodium hydroxide solution (80°C, FIII) in that order. These polysaccharide fractions were further fractionated by ethanol precipitation and gel filtration on the column of Toyopearl HW-65F with 0.3 M sodium hydroxide solution.A strong antitumor activity were found in five xyloglucan-protein complexes, FI-2-a, -b, -c; FII-2-a, and FIII-2-a.Analyses of physico-chemical properties and IR- and NMR-spectra of these active fractions showed that their main components were (1→3)-;(1→6)-β-D-glucopyranans containing a small amount of xylose residues and 2–3% of protein.A mucilaginous polysaccharide (N-PS) prepared from the liquid cultured mycelial broth of the fungus, consisted of (1→6)-;(1→3)-β-D-glucopyranan. Antitumor activities were also found in a subfraction that was purified from N-PS by gel filtration.Also, a strong antitumor activity was fo...