Abstract
Background: Plant biodiversity has great value for science being an inexhaustible source for new bioactive molecules capable of offering environmentally friendly and innovative solutions for various areas of the industry. The scientific community has increased their interest in the study of plant species in the search of new molecules and to determine their mechanisms of action. Plant seeds are natural sources of bioactive compounds, such as carbohydrates, lipids and proteins with special focus on enzymatic inhibitors which protect them against digestive enzymes of phytopathogens and lectins that play an important role on carbohydrate signalization and metabolism during germination. The objective of the present study was to evaluate and describe the protein profile and to test the hemagglutinating, hemolytic and anticoagulant activities, as well as the antitryptic effect of extracts and fractions obtained from seeds of Eucalyptus species. Methods: The crude protein extract was obtained from the seed of Eucalyptus sp. with 0.02 M sodium phosphate buffer, at pH 6.6, and fractionated using ammonium sulfate in order to study its antitryptical properties as well as the capacity of hemagglutination and influence on hemostasis. Results: The crude extract showed a high effectiveness for trypsin inhibition. For hemagglutinating activity, the ammonium sulfate fraction 0-30% presented better activity, while no hemolytic activity was present in the obtained fractions. For anticoagulation assay, the fraction 0-30% showed better results. Conclusions: Taken together, the obtained results demonstrate the biotechnological potential of Eucalyptus sp. seeds, although further study is still necessary to better isolate as well as describe the bioactive compounds.
Highlights
The high biodiversity of plants has led to them becoming of increasing interest to research communities due to their potential for providing new bioactive molecules with new mechanisms of action (Viegas et al, 2006)
The present study had as objective to evaluate the protein profile and to test the hemagglutinating, hemolytic and anticoagulant activities, as well as the antitryptic effect of the crude extracts and fractions obtained from the seeds of Eucalyptus sp
Protein extraction and fractionation of Eucalyptus sp. seeds The seeds used in the present study were donated by the seed bank of the National Forest (Flona) of Nísia Floresta, located in the district of Nísia Floresta, Rio Grande do Norte, Brazil
Summary
The high biodiversity of plants has led to them becoming of increasing interest to research communities due to their potential for providing new bioactive molecules with new mechanisms of action (Viegas et al, 2006). Among the most important studied plant structures, seed extracts have demonstrated potential biological activities such as protease inhibition, hemagglutinating, antibacterial and anticoagulant activities (Otieno & Analo, 2012). The present study had as objective to evaluate the protein profile and to test the hemagglutinating, hemolytic and anticoagulant activities, as well as the antitryptic effect of the crude extracts and fractions obtained from the seeds of Eucalyptus sp. The objective of the present study was to evaluate and describe the protein profile and to test the hemagglutinating, hemolytic and anticoagulant activities, as well as the antitryptic effect of extracts and fractions obtained from seeds of Eucalyptus species. Conclusions: Taken together, the obtained results demonstrate the biotechnological potential of Eucalyptus sp. seeds, further
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