Abstract
Xuebijing (XBJ) injection is an ethnomedicinal formula that has been widely used in the therapy of sepsis in China. However, the underlying theraputic mechanisms remain uninvestigated. In this research, a metabolomic method based on UHPLC-Q-Orbitrap HRMS was applied to make a holistic evaluation of XBJ on septic rats which were induced by the classical cecal ligation and puncture (CLP) operation. The plasma metabolic changes were profiled and evaluated by multivariate analytical (MVA) methods. In the results, a total of 41 differential metabolites were identified between CLP-operated group and sham-operated group, which were mainly involved in amino acid metabolism and lipid metabolism. After pathway analysis, it was finally discovered that the majority of the influenced metabolic pathways caused by sepsis mainly involved in energy metabolism, oxidative stress, and inflammation metabolism. When intervened by XBJ injection, 32 of the 41 disordered metabolites had been adjusted in reverse, which suggested that XBJ could mediate the abnormal metabolic pathways synergistically. In conclusion, the present study systematically investigated the efficacy and its underlying therapeutic mechanisms of XBJ on sepsis, while offering a new insight for the subsequent relevant exploration of other Chinese medicine at the same time.
Highlights
Sepsis is a systemic inflammatory response syndrome (SIRS) that usually caused by polymicrobial bacterial infection (Brent, 2017)
It is clear that the IL-1β, TNF-α, and IL-6 show a higher level in C group than the S group
The inflammatory cytokines level in X group was obviously lower than the C group (P < 0.05), providing a powerful evidence that the XBJ injections had an outstanding anti-inflammatory effect on sepsis
Summary
Sepsis is a systemic inflammatory response syndrome (SIRS) that usually caused by polymicrobial bacterial infection (Brent, 2017). A mass of inflammatory factors (such as TNF-α, IL-1β, and IL-6) is produced, resulting in a more severe systemic inflammatory response that causes damage to Multiple Organ Dysfunction Syndrome (MODS) (Lu et al, 2017). The main drugs used to treat sepsis are glucocorticoids and antibiotics. The key point of treating sepsis is effective and rapid control of the inflammatory reaction. These treatments are only used to relieve the symptoms, which can’t treat sepsis fundamentally and possess a lot of side effects usually. It’s crucial to discover an appropriate drug for this
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