Abstract
Objective To study the suppressive effect of enhancing p27kip1 by knocking down of miRNA-221/222 on the U251 human glioma cell line growth and the possible mechanism. Methods Oligofectamine was used to transfect miRNA-221/222 antisense oligonucleotides to knock down the miRNA-221/221 expression level of U251 human glioma cell line in vitro. Northern blot was conducted to detect the miRNA expression of miRNA-221/222 among different treated groups. The proliferation ability of anti-miRNA-221/222 treated U251 glioma cell was determined by MTT. The cell cycle distribution was detected by cell flow cytometry. Western blot assay was used for p27kip1 expression. Results The expression level of miRNA-221/222 in the anti-miRNA-221/222 group was significantly knocked down than in the other group was determined by northern blot. During the MTT observation, the tumor cell growth was slower in anti-miRNA-221/222 treated group than that in the other treated group. Cell cycle was blocked in G0/G1 phase in the anti-miRNA-221/222 group than in the other group. The expression of p27kip1 was up-regulated in the anti-miRNA-221/222 group by western blot. Conclusion By up-regnlateing p27kip1, anti-miRNA-221/222 suppresses growth of U251 human glioma cell line. Key words: Glioma; miRNA-221; miRNA-222; p27kip1; Cell cycle
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