Abstract
Objectivc To observe effects of antisense human telomerase RNA(hTR) on telomeric length (TRF) and telomerease activity of human gastric cancer cell SGC7901.Methods SGC7901 cell line was transfected with antisense hTR expression vector(pBBS-hTR) by lipofectamine,and expressions of hTR,telomere and telomerase activity in the gene transfected cells were detected by hybridization of nucleic acids and a telomeric repeat amplification protocol(TRAP).Results Clones containing antisense or control plasmids were selected 3 weeks after transfection by HyR selection and either antisense hTR expression had been enhanced or sense hTR expression had been inhibited 72h after transfection by specific stained RNA probe.Telomerase activity and mean TRF length were general low in the gene transfected cells in comparision with those in the control plasmid transfected cells.Conclusion Antisense hTR may either control TRF length by blocking the template region of hTR or serve as a effective target for treating gastric cancer by inhibiting telomerase.
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