Abstract
Amyloid Precursor Protein (APP) is known to be involved in contributing to the beta fragment that is implicated in Alzheimer’s disease (AD). Reducing the expression of APP is one the therapeutic approaches to alleviate AD. We have previously shown that antisense oligodeoxynucleotides (ODNs) to APP mRNA effectively reduce its expression and improve the memory in mice that serve as animal models for AD. For this investigation, we have cloned APP cDNA into a bacterial expression vector, pBAD, and transfected into bacteria. The protein expression could be induced with arabinose and detected by antibody to the tagged V5 peptide. Here we show that the antisense oligonucleotides effectively reduce the expression of APP in bacterial cells by using prokaryotic expression. As the monitoring of the effectiveness of an ODN is faster and simpler compared to eukaryotic expression, such bacterial expression systems may prove to be an improved method of testing pharmaceutical agents aimed to treat AD.
Published Version
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