Antipyretic Activity of AOF Herbal Formulation in Brewer’s Yeast-Induced Pyrexia in Rats

Abstract

The aim of the present study was to investigate the antipyretic activity of AOF herbal formulation by Brewer’s yeast-induced pyrexia in Wistar rats. AOF herbal formulation consists of 13 medicinal plant parts and some of which are reputed for antipyretic effect. The acute oral toxicity was carried out in albino mice according to OECD 423-guidelines. It revealed that there is no toxic sign up to the dose level of 2000 mg/kg body weight. Adult albino rats of either sex (200-250 gm) were divided into five groups containing six in each group for antipyretic study. Before yeast injection, the basal rectal temperature of rats were recorded and after that, the rats were given subcutaneous injection of 10 ml/kg of 15% yeast solution. Rectal temperature of each rat was measured again 19 hours after yeast injection. After confirming that those rats were induced pyrexia, the test drugs and standard drug were administered orally into different groups. Three doses of test drug (1, 1.5 and 2 gm/kg body weight) were used. Paracetamol (150 mg/kg) was administered to standard group. Rectal temperatures were measured at 1, 2, 3, 4 and 5 hours after test drug administration. This herbal formulation (2 g/kg body weight) showed significant reduction of yeast-induced pyrexia in rats at 1 hr, 2 hr, 3 hr and 4 hr after administration of test drug (p<0.001) when compared to the control group. The present results show that AOF herbal formulation possesses a significant antipyretic effect in Brewer’s yeast-induced pyrexia in rats.