ANTIPSEUDOMONAL ACTIVITY OF METABOLIC COMPLEXES OF L. RHAMNOSUS GG AND S. BOULARDII AGAINST THE POLYRESISTENT PATHOGEN IN IN VITRO AND IN VIVO TESTS.

Abstract

The aim: Explore the antimicrobial properties of lactobacilli's metabolites and combination of lactobacilli's and saccharomycetes' metabolites with different concentrations (in vitro) and to test the effectiveness of samples with minimum inhibitory concentration on infected polyresistant strain P. aeruginosa skin wounds (in vivo) for the possibility of creating prophylactic antimicrobial agents. Materials and methods: Metabolic complexes (L. rhamnosus GG and S. boulardi) were obtained by culturing lactobacilli or lactobacilli and saccharomycetes in lactobacilli disintegrates. The sensitivity of Pseudomonas aeruginosa PR (in vitro) to them was determined by the microtechnique of serial dilutions in a liquid nutrient medium. In vivo, 0.9 % sodium chloride solution (control) or lactobacillus metabolic complex (experiment, treatment group) was applied to infected skin wounds or, in addition, immediately before the infection, to the wound and then to infected wounds (experiment, prophylactic-treatment group). Results: There was observed the decrease of the infectious process of skin wounds in the prophylactic-treatment group (3.25-3.4 times; p=0.01 related to control samples) compared with the treatment group (2.05-2.25 times; p=0.02) by the wound healing rate (day 5). The healing rate of control wounds (day 11) coincided with the rates of experimental wounds in the prophylactic-treatment group (day 8), indicating that the use of lactobacilli metabolites promotes the acceleration of healing by almost three days. Conclusions: Metabolic complexes of probiotic microorganisms are promising for construction on their new class of antimicrobials for the effective pharmacoprophylaxis and pharmacotherapy.