Antiproliferative effects of porins extracted from Klebsiella pneumoniae on HL-60, NIH/3T3 and Human foreskin fibroblast cell (HFFC) lines measured by ELISA method

Amir H Al–Shammary,Essam F Al-Jumaily,Nidhal Abdulmohymen

doi:10.24126/jobrc.2010.4.1.89

Amir H Al–Shammary, Essam F Al-Jumaily + Show 1 more

Open Access

https://doi.org/10.24126/jobrc.2010.4.1.89

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Abstract

Approximately, 50% of the dry mass of the outer membrane of gram-negative bacteria consists of proteins, and more than 20 immunochemically distinct proteins (termed outer membrane proteins [OMPs]) have been identified. An identified local strain of Klebsiella pneumoniae was used as a primary source for the isolation and purification of porins. Multiple concentrations of purified porins (5, 10, 15, 20, 25) g/ml were incubated with three different cell lines for (24, 72 , 120) hrs, after the end of the incubation periods, the cells were treated with Cell proliferation ELISA, BrdU (colorimetric) kit to evaluate the antiproliferative effects of porins. The results revealed that porins are potent antiproliferative agent in a time and concentration dependent manner and thus could greatly affect prokaryote-eukaryote interaction as well as the whole inflammatory process resulted after infection with gram negative bacteria.

Highlights

The outer membrane of gram-negative bacteria plays a significant role in a variety of functions; it serves as a diffusion barrier to extracellular solutes and interacts with the bacterial environment
OmpK36 and OmpK35 have been previously described in Klebsiella pneumoniae
They are homologous to OmpC and OmpF, and they are expressed in large amounts in most Klebsiella pneumoniae clinical isolates independently of the isolation source [2]

Summary

Introduction

The outer membrane of gram-negative bacteria plays a significant role in a variety of functions; it serves as a diffusion barrier to extracellular solutes and interacts with the bacterial environment. Results The results of the current study showed that when HL-60 cells were considered, there were significant differences when the control group is compared to the porins treated groups of 15 and 20 μg/ml concentrations and after 24 hrs of incubation.

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Conclusion