Abstract
Leading cause of death world side is because of severe health problems due to cancer. Compounds occurring naturally from plants known as phytochemical are rich sources of anticancer drug development, in depth screening assessments are necessary. The objective of the present study aimed to investigate the mechanistic pathway of antiproliferative activity of ethanolic Andrographis paniculata seed extract against Hepatocellular carcinoma cell (HepG2) cell line. Cell cycle arrest and apoptosis study was performed by Flow cytometry analysis. The expression of certain apoptosis gene associated with cell cycle regulation was conducted by QRT-PCR assay to assess the effect of ethanolic Andrographis paniculata seed extract on HepG2 cells. Andrographis paniculata seed extract inhibited cell proliferation and arrest proliferation on HepG2 cells at G2/M phase when compared to untreated cells. The ethanol extract of Andrographis paniculata seed found to be active cell inhibitor at the time and dose dependent manner. The QRT-PCR assay report reveals that pro-apoptotic genes BCL2 Associated X (BAX), Caspase-3, Caspase-8 genes on both treated and untreated cells. B-cell lymphoma 2 (Bcl-2) is down regulated in treated groups compared to untreated group and Beta actin was used as internal control.
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