ANTI-PROLIFERATION EFFECT AND INDUCTION PPARG EXPRESSION OF HUMAN CERVICAL CANCER CELLS BY COMBINATION OF GRAPE LEAF ETHANOL EXTRACT AND CISPLATIN

Abstract

This study aims to analyze the combination of grape leaf ethanol extract ( Vitis vinifera ) and cisplatin to increase peroxisome proliferator-activated receptor gamma (PPARγ) expression and inhibit HeLa cell proliferation in vitro. This research described a new combination therapy method. This research included the cytotoxicity test, proliferation test with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) Assay, PPARγ expression using immunofluorescence method. The anticancer effects of grape leaf extract (2.5, 5, and 10 mg/mL) were studied through grape leaf phytochemical assays. In this study, the expression of PPARγ increased significantly in grape leaf extract 10 mg/mL + cisplatin. The combination therapy of grape leaf ethanol extract ( Vitis vinifera ) and cisplatin proved to be more effective than single therapy (p < 0.05). Cisplatin with polyphenolic active compounds (phenolics) can inhibit the proliferation of cervical cancer cells. Combination therapy of cisplatin and grape leaf extract inhibited the survival of HeLa cells. Keywords: Grape Leaves, Cell Proliferation, Ethanol Extract, PPARγ, Phenolic Test DOI： https://doi.org/10.35741/issn.0258-2724.58.3.13