Antiplatelet and thrombolytic activity of phenolic-insistent fractions from the new-fangled stem of Ficus benghalensis with concurrent GC-MS analysis

Abstract

Ficus benghalensis is the ingredient of a variety of Ayurvedic herbal formulations for the management of blood-related illnesses. In the current study, the new-fangled stem ethanol extract fractions in chloroform and methanol (CFFB & MFFB) were assessed for antiplatelet, thrombolytic and toxicity studies, as well as for phytoconstituent identification GC/MS was performed. The dried powdered stem bud was extracted with 80% ethanol and successively fractionated by chloroform and methanol (CFFB & MFFB). The anti-platelet, anti-thrombotic, and thrombolytic, activity of CFFB & MFFB were tested in ex vivo mode and toxicity of methanol fraction (MFFB) was tested in in vivo. The chief feasible marker components for antiplatelet activity recognized by GC-MS in the MFFB are Diethyl phthalate, (E)-4-(3-Hydroxyprop-1-en-1-yl)-2-methoxyphenol, 7,9-Di-tert-butyl-1-oxaspiro (4,5) deca-6,9-diene-2,8-dione and trans-Sinapyl alcohol might act as irreversible cyclooxygenase inhibitors like Aspirin. In the study, at 50 ​μg/mL, the antiplatelet activity of CFFB, MFFB, and aspirin was 50.41, 82.19, and 86.34%, and a substantial adjournment in clot development was observed whereas CFFB at different dosages did not exhibit significant outcome on the adjournment of clot formation, antiplatelet, and antioxidant activity. The toxicity examination of MFFB did not confirm any substantial signs of toxicity and mortality up to 1.5 ​g/kg, b.w and non-toxic up to 1.0 ​g/kg, b.w which is capable of the comportment of atherothrombotic ailments. The MFFB exhibited anti-platelet, anti-thrombotic, thrombolytic, and anti-oxidant activity, and capacity to prevent cardiovascular disorders without causing toxicity.