Abstract
The discovery of new molecules for fighting against malaria is still relevant to overcome <i>Plasmodium</i> sp resistance. Phenolic compounds from medicinal plants have shown antiplasmodial properties. In addition, the targets of flavonoids on <i>P. falciparum</i> are multiple. This work aimed to identify the antiplasmodial compounds from methanol extract of <i>Securidaca longepedunculata</i> leaves. The inhibition of β-hematin formation was used to detect antiplasmodial compounds through a bio-guided chromatographic fractionation procedures. W2 strain was inhibited by flavonoids fractions Fc1 and Fb4 with 6.98 and 10.39 µg/mL as IC<sub>50</sub> respectively. Also, fractions of phenol acids have shown good activities on the inhibition of β-hematin formation. The HPLC analyze showed that <i>S. longepedunculata</i> leaves extract contained quercetin, 3-β-quercetin, luteolin, chrysin, isorhamnetin, hyperoside, rutin, gallic acid, ellagic acid, chlorogenic acid, tannic acid and ferulic acid. Among these compounds identified, some had shown antiplasmodial and inhibitory activities on the formation of β-hematin. The antimalarial activity of the leaves of S. longepedunculata would be due in part to phenolic acids and flavonoids. The antiplasmodial activity observed in this work would be due in part to the ability of flavonoids from S. longepedunculata leaves to inhibit the formation of β-hematin. This finding could justify partially the <i>S. longepedunculata</i> uses in malaria treatment in Burkina Faso.
Highlights
The number of malaria cases recorded in 2016 was 216 million, an increase of 5 million compared to the previous year
The ability of crude extract and 12 fractions to inhibit the American Journal of BioScience 2020; 8(1): 1-5 formation of β-hematin was summarized in the Figure 1
The fractionation by flash chromatography of one gram of the mixture of F7 and F8 gave twenty-seven (27) sub-fractions (F1’-F27’). According to their TLC chromatographic profile, these sub-fractions were grouped into 4 groups which were Fa (F1’-F’7), Fb (F’8), Fc (F9'-F’25) and Fd (F26'- F27') which were submitted to the β-hematin inhibition assay and the result was indicated by the Figure 2
Summary
The number of malaria cases recorded in 2016 was 216 million, an increase of 5 million compared to the previous year. Effective treatment of Malaria remains a challenge for modern medicine as part of its eradication. The complexity of development cycle and the ability of P. falciparum to resist against new antiplasmodial molecules posed a threat to the global health system, especially in sub-Saharan Africa [1]. One of alternative to conventional medicine, is to promote heavily the use of medicinal plants for the malaria treatment [3, 4]. These plants have always constituted an investigation source to research new interest compound [5]. Molecules belonging to different chemical groups with very interesting antiplasmodial activities have been isolated from antimalarial plants [6, 7]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
