Antiphospholipid antibodies effect over trophoblast cell function and pharmacological modulation

Abstract

Event Abstract Back to Event Antiphospholipid antibodies effect over trophoblast cell function and pharmacological modulation Angela M. Alvarez1, Yonhatann Ramírez1, Sebastián Sanmartín2, Udo Markert3 and Angela Cadavid1* 1 University of Antioquia, Colombia 2 University of Valparaíso, Chile 3 University Hospital Jena, Germany Since antiphospholipid antibodies (aPL) are involved in impaired placental development in women with antiphospholipid syndrome (APS), the aim of this study was to determine the effect of aPL on the first trimester trophoblast cell line HTR-8/SVneo function and on the activation of the signal transducer and activator of the transcription 3 (STAT-3) pathway. IgGs purified from women with aPL and clinical manifestations of pregnancy morbidity and/or vascular thrombosis (PM and PM/VT groups), women with pregnancy morbidity without aPL (PM/aPL-), and also healthy women as controls (NHS) were used. First, the aPL binding to trophoblast cells was assessed by immunohistochemistry. Then, trophoblast viability, proliferation and invasion were evaluated. The effects of aspirin, enoxaparin and aspirin-triggered lipoxins (ATL) on antiphospholipid antibody-decreased trophoblast invasion were also evaluated. IgG from women with APS bound strongly to the first trimester extravillous trophoblast cell line HTR-8/SVneo. Reduced cell viability and STAT-3 activation were induced by IgG from women of PM group. On the other hand, the mitochondrial membrane integrity was reduced by IgG from women of PM/VT group, which in contrast induced an increase in proliferating trophoblast cells. Both PM and PM/VT IgG induced a decrease in trophoblast invasion. Aspirin, enoxaparin, and ATL restored the aPL-decreased invasion. Addition of the ATL receptor antagonist Boc-2, reversed the ATL-restored invasion. In conclusion, IgG from women with PM or PM/VT induced different effects on trophoblast cells. Trophoblast invasion was clearly altered but, interestingly, it was restored with enoxaparin, aspirin and, as shown for the first time with ATL. Additionally ATL has a receptor-mediated effect in trophoblast cells as demonstrated by addition of Boc-2. Acknowledgements CODI- Universidad de Antioquia Grant 91515; Sustainability Strategy Grupo Reproducción 2014-2015; Scientific International Cooperation: Colciencias Grant 91567 and CONICYT Grant PCCI 130017; AMA is fellow from Colciencias. Keywords: Antiphospholipid Syndrome, Antiphospholipid antibodies, Trophoblasts, STAT-3, trophoblast invasion Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Autoimmunity Citation: Alvarez AM, Ramírez Y, Sanmartín S, Markert U and Cadavid A (2015). Antiphospholipid antibodies effect over trophoblast cell function and pharmacological modulation. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00228 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 May 2015; Published Online: 14 Sep 2015. * Correspondence: MD, PhD. Angela Cadavid, University of Antioquia, Medellín, Colombia, angela.cadavid@udea.edu.co Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Angela M Alvarez Yonhatann Ramírez Sebastián Sanmartín Udo Markert Angela Cadavid Google Angela M Alvarez Yonhatann Ramírez Sebastián Sanmartín Udo Markert Angela Cadavid Google Scholar Angela M Alvarez Yonhatann Ramírez Sebastián Sanmartín Udo Markert Angela Cadavid PubMed Angela M Alvarez Yonhatann Ramírez Sebastián Sanmartín Udo Markert Angela Cadavid Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.