Antiphospholid antibodies regulate the expression of trophoblast cell adhesion molecules

Abstract

Objective: To examine the effect of antiphospholipid antibodies on trophoblast expression of adhesion molecules. Design: Primary cytotrophoblast cell cultures. Setting: Department of Obstetrics and Gynecology, Catholic University, Rome, Italy. Patient(s): Five normal pregnant women underwent uncomplicated vaginal delivery at 36 weeks of gestation. Intervention(s): IgG antibodies were isolated from a patient with antiphospholipid syndrome and from a normal control subject, using protein-G Sepharose columns. Cytotrophoblast cells were dispersed in bicarbonate buffer containing trypsin and DNAse I. Main Outcome Measure(s): We investigated the effects of antiphospholipid antibodies on trophoblast adhesion molecules (α1 and α5 integrins, E and VE cadherins), both at the protein and mRNA levels. Result(s): The α1 and α5 integrins were present in trophoblast cells from 24 hours of culture. Treatment with IgG that were obtained from the patient with antiphospholipid syndrome significantly decreased α1 integrin and increased α5 integrin at both the mRNA and protein levels. Furthermore, IgG with antiphospholipid antibodies activities induced VE-cadherin down-regulation and the E-cadherin up-regulation at protein and mRNA levels compared with control IgG or untreated cells. Conclusion(s): The results suggest that the inadequate trophoblastic invasion, induced by antiphospholipid antibodies, can be the result of abnormal trophoblast adhesion molecules expression.