Abstract
Background and aim : Ischemia-reperfusion (I/R) in the intestines activates leukocytes and reactive oxygen species (ROS) and leads to lipid peroxidation and DNA damage. Rosehips have a high content of polyphenols and might prevent lipid peroxidation. Some Lactobacillus species are capable of degrading polyphenols to simpler and non-toxic constituents, sometimes with enhanced antioxidative capacity. Methods : A mixture of eight polyphenol active Lactobacillus strains (LAB) were administered in feed together with rosehips of Rosa canina (RC) or Rosa pimpinellifolia (RP) to mice to clarify the influence on I/R-induced injury of the intestinal tract. I/R injury was induced by holding the superior mesenteric artery clamped for 30 min followed by reperfusion for 240 min. Tissue and content from colon and cecum were analyzed. Results : Administration of LAB or RP signifi cantly decreased malondialdehyde (MDA) levels in colonic tissue (p=0.007 and p=0.035, respectively). RC contained signifi cantly higher concentrations of monomer and dimer aglycones, glycosides, and catechin than RP, while cyanidin-3-O-glycoside was signifi cantly higher in RP. There was a tendency towards higher values of phenolics in the mice fed either of the two rose species in combination with bacterial mixture than the mice fed the rose species alone. Total antioxidative capacity and total phenolic content were higher in the groups fed rosehips in combination with LAB than rosehips alone, although these differences were not signifi cant. Conclusion : LAB showed metabolic activity towards polyphenols in rosehips. There is an indication that LAB together with rosehips, especially R. pimpinellifolia , may prevent and suppress I/R injury in the intestines. Key words: Ischemia-reperfusion, gut, rosehip, Lactobacillus plantarum
Highlights
The gut is a complex and dynamic ecosystem with a high bacterial load
Animals were randomly divided into the following seven groups, with seven mice in each group: I/R control group, with ischemia/reperfusion injury, fed soft standard chow; sham group, without ischemia/ reperfusion injury, fed soft standard chow; RC group, fed standard chow supplemented with Rosa canina (RC); RCϩLAB group, fed standard chow supplemented with R. canina and a mixture of eight different polyphenol active Lactobacillus strains (LAB); RP group, fed standard chow supplemented with Rosa pimpinellifolia (RP); RPϩLAB group, fed standard chow supplemented with R. pimpinellifolia and a mixture of eight different polyphenol active LAB; LAB group, fed standard chow supplemented with a mixture of eight different polyphenol active LAB
Rosehips used in the experimental diets originated from two species of roses grown at Balsgård (Sweden), i.e. Rosa pimpinellifolia (RP) and Rosa canina (RC)
Summary
The gut is a complex and dynamic ecosystem with a high bacterial load. It is an immune organ, represented by the gut-associated lymphoid tissue or GALT [1]. Dysfunction of the mucosal barrier can result in translocation of bacteria, for example, endotoxincontaining bacteria such as Enterobacteriaceae out to extraintestinal sites. This can lead to sepsis, shock, and multiple organ failure (MOF) [2]. Ischemia-reperfusion (I/R) in the intestines activates leukocytes and reactive oxygen species (ROS) and leads to lipid peroxidation and DNA damage. Methods: A mixture of eight polyphenol active Lactobacillus strains (LAB) were administered in feed together with rosehips of Rosa canina (RC) or Rosa pimpinellifolia (RP) to mice to clarify the influence on I/R-induced injury of the intestinal tract. There is an indication that LAB together with rosehips, especially R. pimpinellifolia, may prevent and suppress I/R injury in the intestines
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
