Antioxidative Potential of Consciousness Energy Healing Treatment on HepG2 Cells and DMEM after Oxidative Stress Induced by Hydrogen Peroxide

Mahendra Kumar Trivedi,Gopal Nayak,Trivedi Trivedi,Sambhu Charan Mondal,Alice Branton,Snehasis Jana,Jie Yin

doi:10.14302/issn.2471-2140.jaa-18-2400

Abstract

Antioxidants can reduce oxidative stress in cells is used for the treatment of several disorders such as cancer, cardiovascular, and inflammatory diseases. The present study was evaluated the antioxidant potential of the Consciousness Energy Healing (The Trivedi Effect®) Treated human hepatoma cell line (HepG2) and Dulbecco's Modified Eagle Medium (DMEM) for the assessment of cell viability under hydrogen peroxide-induced oxidative stress. The Biofield Energy Treated HepG2 cells group was maintained for 23 days under standard conditions. On the next day, the cells were challenged with 1 mM of H2O2 for the generation of oxidative stress. The ability of the Biofield Energy Healing Treatment to protect from the oxidative stress was determined by MTT cell viability assay and compared with the negative control group. The percentage of cell viability was significantly (p≤0.001) increased by 13.6% in the Biofield Energy Treated DMEM group; while altered by 3.2% in the Biofield Energy Treated HepG2 cells group compared to the negative control group. Overall, the Biofield Energy Treated DMEM showed a better antioxidative protection against oxidative stress than HepG2 cells group, which was induced by H2O2. Therefore, the results envisaged that The Trivedi Effect®- Biofield Energy Healing Treatment has an impact on the protection of various vital organs from oxidative stress; which might be helpful in the development of powerful/energized growth medium for the accelerated study with a cost-effective manner.

Highlights

Oxidative stress, is defined as the imbalance between the generation of reactive oxygen species (ROS) and antioxidant defense system
G5 referred as the Biofield Energy Treated HepG2 cells in addition to the untreated Dulbecco's Modified Eagle Medium (DMEM) supplemented with 1% fetal bovine serum (FBS) and H2O2 (1 mM)
The percent cell viability was significantly (p≤0.001) increased by 13.6% in the Biofield Energy Treated DMEM group; while decreased by 3.2% in the Biofield Energy Treated HepG2 cells group compared to the negative control group

Summary

Introduction

Oxidative stress, is defined as the imbalance between the generation of reactive oxygen species (ROS) and antioxidant defense system. It has been well known that ischemia (an inadequate blood supply to an organ) initiates the noxious generation of ROS, the re-oxygenation process is responsible for the production of ROS, activation of complement system, and inflammatory response [1] Antioxidant enzymes, such as catalase (CAT) and superoxide dismutase (SOD) that detoxify H2O2 and superoxide, are highly potent and specific agents to the ROS-induced injury [2]. The liver is the main detoxifying organ of the human body, human hepatocyte (HepG2 cell line and human hepatoma) was used as the test system in the present study to assess the protective effect of Biofield Energy Treatment (The Trivedi effect®) against hydrogen peroxide-induced oxidative damage

Materials and Methods

Results and Discussion

Conclusions