Abstract
Evaluation of the antioxidant potential of methanol extract of Chenopodium botrys L. (Amaranthaceae) collected from six different locations in Republic of Macedonia was performed. Several methods were used for testing the antioxidative activity: 1) 2,2-diphenyl1-picrylhydrazyl (DPPH) radical scavenging assay, 2) ferric reduction power assay (FRAP), 3) inhibition of H2 O2 activity, 4) non-sitespecific hydroxyl radical-catalyzed 2-deoxy-D-ribose degradation (NSSOH) and 5) site-specific hydroxyl radical-catalyzed 2-deoxy-D-ribose degradation (SSOH). The IC50 values ranged from 0.26-3.10 mg/mL, 3.01-12.71 mg/mL and 2.60-12.29 mg/mL, for DPPH, NSSOH and SSOH assays, respectively. The H2 O2 inhibition activity and the ferric reducing power capacity were from 28.84-46.56% and 26.14- 43.40%, respectively. The obtained data establish the antioxidant potency in concentration-dependent manner. Additionally, total phenols (TPC) and total flavonoid content (TFC) were determined. The estimated values ranged from 27.77-71.25 mg GAE/g DW and from 7.35- 16.33 mg QE/g DW, respectively
Highlights
Oxidative stress plays an important role in the etiology of chronic diseases such as cancer, atherosclerosis and diabetes as well as age-related neurological degenerative disorders
Several methods were used for testing the antioxidative activity: 1) 2,2-diphenyl1-picrylhydrazyl (DPPH) radical scavenging assay, 2) ferric reduction power assay (FRAP), 3) inhibition of H2O2 activity, 4) non-sitespecific hydroxyl radical-catalyzed 2-deoxy-D-ribose degradation (NSSOH) and 5) site-specific hydroxyl radical-catalyzed 2-deoxy-D-ribose degradation (SSOH)
In the aerial parts of C. botrys, the flavon chrysoeriol as well as flavonoids: salvigenin, sinensetin, hispidulin, quercetin and their derivatives were identified, which were related to a potential antioxidant activity (Kokanova-Nedialkova et al, 2009)
Summary
Oxidative stress plays an important role in the etiology of chronic diseases such as cancer, atherosclerosis and diabetes as well as age-related neurological degenerative disorders. The genus Chenopodium comprises numerous members of perennial and annual species, which occur everywhere in the world. They could grow as aromatic or non-aromatic herbaceous plants or as shrubs and small trees (Fuentes-Bazan et al, 2012). Concerning literature data, C. botrys contains flavonoids, alkaloids, terpenes and essential oil (Kokanova-Nedialkova et al, 2009). In the aerial parts of C. botrys, the flavon chrysoeriol as well as flavonoids: salvigenin, sinensetin, hispidulin, quercetin and their derivatives were identified, which were related to a potential antioxidant activity (Kokanova-Nedialkova et al, 2009)
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