Abstract
Oxidative stress and antioxidative defense system activity were studied in buckwheat leaves after complete submergence and re-aeration. The levels of H2O2 and lipid peroxidation were found to be significantly higher in stressed than in untreated buckwheat leaves. Enzymes catalyzing the degradation of H2O2 and peroxides were shown to participate actively, whereas superoxide dismutase did not take part in the buckwheat leaf response to flooding stress. The most prominent increase in antioxidative enzyme activities was noticed upon return to air, when the strongest oxidative stress occurred and the need for antioxidative defense was the greatest.
Highlights
Submergence refers to the situation when floodwaters rise to levels that keep shoots completely under water
In this study we report that both complete submergence and re-aeration cause oxidative damage in buckwheat leaves and induce their antioxidative defense system
Brown precipitation products indicating the accumulation of H2O2 in the leaf tissue were more pronounced in the re-aerated leaves than in those subjected to submergence only (Fig. 1)
Summary
Submergence refers to the situation when floodwaters rise to levels that keep shoots completely under water. At very low light intensity, mitochondria are the main source of reactive oxygen species (ROS) due to the activity of the mitochondrial electron transport chain (Skutnik and Rychter, 2008). In the absence of O2, which is the terminal acceptor in the mitochondrial respiratory chain, the ATP level decreases and is synthesized mainly by alcoholic fermentation (Yordanova and Popova, 2002). Due to their close dependence on O2 homeostasis for normal functioning, mitochondria may play a crucial role in sensing changes in environmental O2 levels (Dat et al, 2004). The depletion of ATP, increased reduction state of the cell environment and hyper-polarization of
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