Antioxidative effect of dietary tea catechins on lipid oxidation of long-term frozen stored chicken meat

Abstract

The antioxidative effect of dietary tea catechins (TC) supplementation at levels of 50, 100, 200 and 300 mg kg −1 feed on susceptibility of chicken breast and thigh meat to lipid oxidation during frozen (−20°C) storage for 9 months was investigated. Day-old chickens (Cobb 500, n=200) were randomly divided into six groups. Chickens were fed a basal diet containing 20 mg α-tocopheryl acetate kg −1 feed as control, or a vitamin E supplemented diet (basal diet plus 200 mg α-tocopheryl acetate kg −1 feed), or TC supplemented diets (basal diet plus 50, 100, 200 or 300 mg TC kg −1 feed) for 6 weeks prior to slaughter. Lipid oxidation (TBARS) was assessed after 0 and 10 days of refrigerated display (4°C) following 1, 3, 6, and 9 months of frozen (−20°C) storage. TC supplementation at all concentrations showed antioxidative effects for both breast and thigh chicken meat during the 9 months of frozen storage compared to the control sample. TC supplementation at levels of 200 and 300 mg kg −1 feed were more effective ( P<0.05) in delaying lipid oxidation in all meat samples compared to the control. TC supplementation at a level of 200 mg kg −1 feed showed antioxidant activity equivalent to α-tocopheryl acetate fed at the same level up to 3 months of frozen storage. For long-term frozen storage up to 9 months, however, TC supplementation at 300 mg kg −1 feed was required as a replacement for α-tocopheryl acetate at a level of 200 mg kg −1 feed. The results obtained showed a long-term antioxidative effect exhibited by dietary tea catechins on chicken meat during frozen storage and demonstrated that tea catechins are effective alternatives to vitamin E as natural dietary antioxidants.