Antioxidative and anti-inflammatory effect of ethanol extracts from Magnolia kobusDC on LPS-induced in vitroand in vivomodel

Abstract

Abstract Magnolia kobusDC (M. kobus) has biological effects such as antioxidant, anti-inflammatory and antibacterial properties in its flower, bud, and stem bark extracts. Despite of diverse effectiveness of M. kobus, the pharmacological effects supporting inhibition of oxidation and inflammation on acute lung inflammation states have not been studied yet. The purpose of this study was to evaluate the pharmacological activity of M. kobusleaf ethanol extract (MkDE) by antioxidant and anti-inflammatory effects on acute lung inflammatory diseases. The antioxidant and anti-inflammatory effects of MkDE was evaluated using ROS detection assay, NO assay, qPCR, ELISA, and Western blot on LPS-induced macrophages. The therapeutic effect of MkDE was investigated in vivooxidative and inflammatory responses by histological observations, qPCR, ELISA, and Western blot using the LPS-induced acute lung inflammation mouse model. MkDE decreased ROS production and protein of Nrf2. MkDE showed a protective effect against H 2O 2as well as a decrease in NO production. MkDE reduced the levels of pro-inflammatory cytokines and chemokine, the activation of COX-2 and iNOS, and NF-κB translocation in LPS-induced macrophage. The intraperitoneal injection of MkDE showed to decrease the lung/body weights, infiltrated inflammatory cells into the lung alveoli, pro-inflammatory mediators, and serum released proteins. Furthermore, MkDE inhibited the activation of Nrf2, COX-2 and NF-κB in LPS-induced acute lung tissue. MkDE exhibits the pharmacological effect by reducing oxidative and inflammatory mediators on LPS-induced macrophages and acute lung inflammation mouse model. Taken together, MkDE might be used to treat acute lung disease.