Antioxidative Activity of Tithonia Diversifolia Extract in Streptozotocin-Induced Diabetic Rats.

Abstract

Diabetes mellitus (DM) is metabolic disease characterized by high blood glucose. Tithonia diversifolia (TD) is a traditional herbal plant that contains anti-oxidative substances to reduce toxicity by free radical molecules. This study is aimed to analyzed effect of Tithonia diversifolia extract on diabetic rats. Dried leaves of Tithonia diversifolia plant (Balittro,Bogor) were sieved and macerated using 96% ethanol. TD leaves extract was dissolved in 0.1% sodium carboxymethyl cellulose (CMC-Na). Twenty-four male Wistar rats (Rattus norvegicus) were allocated into four groups; The control received normal saline (P0). The positive control received 0.1% CMC-Na (P1), the treatment received 100 mg/kg bw of TD extracts (P2) and cathecin 10 mg/kg bw (P3) respectively for 7 days. Bloods were collected for analysis of blood glucose (BG) and alkaline phospathase (ALP). The levels of MDA and SOD concentrations were conducted by Sandwich-ELISA. Based on result showed that feeding TD extract significantly could decrease the level of BG and ALP concentration compared to the positive control group (p<0.05). Level of MDA was decreased meanwhile level of SOD concentration significantly (p<0.05) on treatment group. It was concluded that administration of TD extract could restore normal blood glucose by antioxidant effect on diabetic rats.