Antioxidative activity of the polyphenols from the involucres of Castanea mollissima Blume and their mitigating effects on heat stress

Abstract

Polyphenols extracted from plants have multiple functions in animal production. To explore new sources of tannin-rich extracts, which have potential benefits for animal health, this study focused on the effects of polyphenolic extracts from involucres of Castanea mollissima Blume (PICB) on heat-stressed broilers. In vitro experiments were first performed using intestinal cryptlike epithelial cell line-6 (IEC-6) cells to evaluate the effects of PICB on cell proliferation and antioxidative parameters under normal and heat-stress conditions. Then in vivo experiments were carried out with 2 trials: in trial 1, 400 one-d-old male Arbor Acres (AA) broilers were randomly assigned to 5 groups (4 replicates/group, 20 chicks/replicate): group 1 was a normal control group fed the basic ration; groups 2 to 5 were fed the basic ration supplemented with 0.2% vitamin C and 0.2%, 0.3%, and 0.4% PICB, respectively. Trial 1 lasted 42 d, and growth performance was monitored every week. At the end of the trial, the chicks were sacrificed and sampled. In trial 2, 400 twenty-eight-d-old chicks were randomly assigned to 5 groups as described in trial 1. After 1 week of adaptation, heat stress was applied for 7 consecutive days. On days 3 and 7 of heat stress, the chicks were sacrificed and sampled. The results showed that PICB could stimulate IEC-6 cell proliferation and had strong in vitro antioxidant activity. PICB had no effect on the growth performance and carcass parameters of AA broilers in trial 1, whereas in trial 2, group 4 saw improved growth performance and antioxidant activity compared to the first three groups (P < 0.05). In conclusion, PICB had no effects on the growth performance of IEC-6 cells and AA broilers under normal conditions, whereas it could mitigate heat-stress effects on the growth performance and antioxidant activity of IEC-6 cells and AA broilers, implying that PICB could be used as a suitable additive to improve animal production under heat-stress conditions.