Abstract
This study investigated the polyphenol content, antioxidant activity, and inhibition ability of mushroom tyrosinase and melanogenesis of Dendrobium tosaense (DT) extract. Ground DT was extracted using deionized water (W) or 50% ethanol (50E) at room temperature (RT) or 50 °C (50T) for 20 min. The 50T + 50E extract exhibited the highest total phenol content 47.0 ± 4.0 mg gallic acid equivalent/g DT extract, the highest level of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging 66.0 ± 3.0 mg Trolox equivalent/g DT extract, and the highest reducing power 12.00 ± 0.50 mg vitamin C equivalent/g DT extract. The RT + W extract had the highest total flavonoid content 110.0 ± 3.0 mg quercetin equivalent/g DT extract. The RT + 50E extract had the lowest half maximal inhibitory concentration 1.30 ± 0.00 mg/mL for 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging, and the lowest half maximal inhibitory concentration 6.40 ± 0.30 mg/mL for mushroom tyrosinase inhibition activity. DT extracts, especially RT + W and 50T + W, exhibited potent inhibitory effects on melanogenesis of B16/F10 cells. These results demonstrated the application potential of DT extract for skincare.
Highlights
The origins of orchids (Orchidaceae) can be traced back 120 million years [1]
In the methylthiazol tetrazolium (MTT) assays (Figure 8), all Dendrobium tosaense (DT) extracts and 1 mg/mL of kojic acid exhibited no cytotoxicity to the B16/F10 cells
The viability of cells treated with 20 μL of DT extract with a quintuple dilution was determined using a methylthiazol tetrazolium (MTT) assay
Summary
The origins of orchids (Orchidaceae) can be traced back 120 million years [1]. Dendrobium, known as “Shihu” in China, is the second largest genus in Orchidaceae [1]. In addition to exhibiting antitumor, analgesic, and antipyretic properties is a type of bibenzyl compound [12] that functions as an antioxidant and contributes to inhibition of [10,11]. Contributes torich inhibition of cataractogenesis asexhibit well antitumor as exhibiting anti-lung-cancer and Dendrobium is in active polysaccharides, which and antioxidation properties properties[14]. Few reports have evaluated DT extracts that have been prepared throughTherefore, various the aim of the present study determine the polyphenol antioxidant activity, mushroom methods. Thewas aimtoof the present study was contents, to determine the polyphenol contents, tyrosinase inhibition ability and melanogenesis inhibition of DT obtained using various antioxidant activity, mushroom tyrosinase inhibition ability andsamples melanogenesis inhibition of DT solvent extraction methods.
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