Abstract
BackgroundTang Bi Kang (TBK) is a traditional Chinese medicine granule. It has been shown to have effects on nerve conduction velocity deficits, blood-related factors and oxidative stress. This study was undertaken to evaluate proposed antioxidative and anti-inflammatory activity of Tang Bi Kang in rats with diabetic peripheral neuropathy (DPN).MethodsDPN was induced in male Wistar rats by intraperitoneal administration of streptozocin (STZ) (60 mg/kg.b.w) for 8 weeks. Fasting blood glucose (FBG) levels were measured in the blood obtained by clipping the tails of the rats. Tail-flick tests were conducted with a tail-flick analgesic meter. Motor and sensory nerve conduction velocities (MNCV and SNCV) of sciatic nerve were measured directly at two sites using a Functional Experiment System. Oxidative stress makers such as malondialdehyde (MDA), superoxide-dismutase (SOD) and glutathione peroxidase (GSH-Px), inflammatory cytokines such as interleukin (IL)-6, and tumour necrosis factor (TNF)-α were estimated. The statistical analysis of results was carried out using Student t-test and one-way analysis of variance (ANOVA), followed by least-significant difference post hoc with SPSS.ResultsThe administration of TBK for 4 weeks in DPN rats resulted in a significant decrease in FBG levels compared to untreated DPN rats. There was a significant increase in MNCV and SNCV in the DPN rats compared to untreated DPN rats. Serum level of MDA was significantly reduced while the activities of SOD and GSH-pX were significantly increased in the TBK treated DPN rats. TBK prevented DPN-induced increase in the serum levels of IL-6 and TNF-α.ConclusionThe results of this study demonstrate that the therapeutic effect of TBK on DPN rats may be associated with the antioxidative and anti-inflammatory responses.
Highlights
Tang Bi Kang (TBK) is a traditional Chinese medicine granule
Enhanced nuclear factor-κB (NF-κB) activity during the hyperglycemic state is associated with excess production of proinflammatory cytokines such as IL-6, Tumour necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2) and induces the generation of nitric oxide synthase
A significant increase in tail flick latency was observed after 8 weeks of STZ induction (P < 0.01), and Motor nerve conduction velocity (MNCV) and Sensory nerve conduction velocity (SNCV) were significantly reduced in the STZ-treated rats compared to the normal rats (P < 0.01) (Table 3)
Summary
Tang Bi Kang (TBK) is a traditional Chinese medicine granule. It has been shown to have effects on nerve conduction velocity deficits, blood-related factors and oxidative stress. This study was undertaken to evaluate proposed antioxidative and anti-inflammatory activity of Tang Bi Kang in rats with diabetic peripheral neuropathy (DPN). Enhanced NF-κB activity during the hyperglycemic state is associated with excess production of proinflammatory cytokines such as IL-6, TNF-α, cyclooxygenase-2 (COX-2) and induces the generation of nitric oxide synthase (iNOS). These proteins and enzymes are mediators prerequisite for the initiation and amplification of inflammatory processes in neuronal cells [12]. Reduced Nrf activity results in impaired antioxidative defense, which is characterized by decline in SOD, catalase and glutathione levels It decreases the production of detoxifying enzymes leading to nitrosative and oxidative stress [10]
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