ANTIOXIDANTS STATUS OF PATIENTS WITH CHRONIC KIDNEY DISEASE ON HEMODIALYSIS AND CONSERVATIVE THERAPY - A COMPARATIVE STUDY

Abstract

Background: There is growing evidence from experimental and clinical studies that oxidative stress is a potentially important source of morbidity and mortality in patients with chronic kidney disease (CKD). This study was carried out to determine the selected antioxidant status of patients with CKD involving forty-ve (45) subjects consisting of 15 patients on conservative therapy and another 15 patients on haemodialysis (experimental groups) and also 15 apparently healthy volunteers as control. Ten (10) mls of blood samples Methodology: were obtained from the subjects into heparinized bottles for determination of selected antioxidants such as serum retinol, α-tocopherol and lycopene using high performance liquid chromatography (HPLC) method and ascorbic acid and selenium using atomic absorption spectrophotometry method. The serum retinol, Results: (57.31±5.97 µg/dl and 59.19±7.71 µg/dl), serum ascorbic acid, (34.62±7.06 mmol/l and 37.16±7.26 mmol/l), serum α-tocopherol, (1.05±0.23 mg/dl and 1.11±0.31 mg/dl) and lycopene, (36.34±7.67 µg/dl and 37.48±6.76 µg/dl) of the patients on conservative therapy and haemodialysis respectively were signicantly higher than the values 44.62±7.38 µg/dl, 29.41±7.41 mmol/l, 0.63±0.13 mg/dl and 23.09±4.24 µg/dl observed in the control respectively (p<0.05). The serum selenium of the patients with CKD was not signicantly different from the control (p>0.05). Conclusion: The diet of patients with CKD should be planned individually and tailored towards their biochemical information to maintain nutritional status and avoid nutrient toxicity.