Abstract
A practical approach for identifying antioxidants from volatile MRPs was developed based on the comparison of volatile MRPs before and after their reaction with DPPH radical by GC–MS analysis. Using the approach, 2,5-dimethyl-4-hydroxy-3(2H)furanone (DMHF) and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) were identified as the contributors to the antioxidant activity of volatile MRPs from Glc-His system while DMHF, 2-hydroxy-3-methyl-2-cyclopenten-1-one (CP) and 3-acetyl-1,6-dimethyl-2,4(1H,3H)pyridinedione (ADMP) as the contributors to the antioxidant activity of volatile MRPs from Glc-Tyr system. To test the effectiveness of the approach, seven compounds (DMHF, DDMP, CP, 2-furanmethanol, Furfural, 2-methylpyrazine, 2-acetylpyrrole) were investigated individually for their DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power by UV–Vis analysis, which further confirmed the antioxidant activity of DMHF, DDMP and CP. Thus, the approach could be used to identify antioxidants from other volatile mixture. Besides, the interaction analysis denied the existence of the synergistic effect between each two compounds from above seven compounds.
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