Abstract
Recently, research on the biological effects of microplastics (MPs) has grown exponentially. However, effects of MPs on freshwater fishes and the mechanisms of the biological effects of MPs were limited. So, the purpose of the current study was to clarify the effects of microplastics on oxidative stress response, DNA fragmentation, and proteinogram of the early juvenile stage of Nile Tilapia (Oreochromis niloticus). The fishes were assigned into four groups: one control, three MPs-exposed groups as 1 mg/L of MPs, 10 mg/L of MPs, and 100 mg/L of MPs respectively for 15 days and 15 days of recovery. The activities of superoxide dismutase, catalase, total peroxides, and oxidative stress index (OSI), as well as lipid peroxidation and DNA fragmentation, increased in groups exposed to MPs compared to the control group in a dose-dependent manner. In contrast, the activity of total antioxidant capacity decreased in groups exposed to MPs compared to the control group in a dose-dependent manner. The electrophoretic pattern of muscle proteins revealed alteration in the proteinogram in the MPs-exposed groups compared to control. After the recovery period, the activities of superoxide dismutase, catalase, total peroxides, total antioxidant capacity, lipid peroxidation, DNA fragmentation, and the electrophoretic pattern of muscle proteins returned to normal levels in 1 mg/L of MPs-exposed group. Combined with our previous work, these results suggest that MPs cause the overproduction of reactive oxygen species (ROS) and alters the antioxidants parameters, resulting in oxidative stress and DNA damage. The present study fosters a better understanding of the toxic effects of MPs on Tilapia as a freshwater model.Graphical
Highlights
Highlights Microplastics (MPs) were induced antioxidant alterations in tilapia early juvenile. DNA damage was reported in the tilapia after exposure to microplastics (MPs) The effect of microplastics (MPs) was recoverable in concentrationdependent.Responsible editor: Philippe GarriguesDepartment of Zoology, Faculty of Science, Al-AzharUniversity (Assiut Branch), Assiut 71524, EgyptDepartment of Zoology, Faculty of Science, Sohag University, Sohag 8562, EgyptDepartment of Zoology, Faculty of Science, Assiut University, Assiut 71516, EgyptRecently, there is increasing sensibility of the harmful effects of microplastics due to their extensive use in commercial, industrial, and medicinal applications (Lusher et al 2017)
The present study aimed to examine the effects of microplastics on oxidative stress response, DNA fragmentation, and proteinogram of the popular fish (Oreochromis niloticus) early juvenile
Lipid peroxidation and DNA fragmentation showed significant increase (P < 0.05) after exposure to 1, 10, and 100 mg/L of microplastics for 15 days compared to the control group (Figs. 1 and 2)
Summary
Responsible editor: Philippe GarriguesRecently, there is increasing sensibility of the harmful effects of microplastics due to their extensive use in commercial, industrial, and medicinal applications (Lusher et al 2017). It is known that exposure of fish to different pollutants in the water ecosystem can prompt the surfeit of reactive oxygen species (ROS) which causes harmful effects to macromolecules of cells (Sureda et al 2006) because microplastics can be digested by fish and causes some effects (de Sá et al 2018; Jovanovic 2017). The fish have an intricate antioxidant system which comprises superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GPx), which encounter the oxidative damage of reactive oxygen species (ROS) (Sayed and Soliman 2018; Soliman et al 2019). Oxidative stress index (OSI) is a comparative indicator illustrating the interaction among the freeradical inducing agents and their antioxidants system (Fazio et al 2015; Sayed and Abul Khalil 2016). Malondialdehyde (MDA) is regarded as a bioindicator of the lipid peroxidation
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