Antioxidants and Hepatoprotective Study of a Purified Bauhinia Variegate Leaves and Flowers Against Carbon Tetrachloride-Induced Toxicity in Experimental Rats

Abstract

This study was designed to investigate the hepatoprotective activity and antioxidant enzymes of purified Bauhinia variegate leaves extract and purified flowers extract were administered (200 mg/kg, orally once daily) to reduce the effect of carbon tetrachloride-damage in rat's liver for three weeks. Thereafter, the levels of some serum biochemical factors such as alanine amino transferase, aspartate amino transferase, alkaline phosphatase, and the activity of three different antioxidant enzymes (glutathione, superoxide dismutase, and catalase) were investigated. Liver homogenate can used to estimated antioxidant parameters: glutathione, superoxide dismutase and catalase. The purified Bauhinia variegate leaves and purified flowers significantly (p < 0.05) inhibited the carbon tetrachloride-induced increase in alanine aminotransferase (149.0± 4.40 and 133.08±6.84) unit/L, aspartate aminotransferase (114±9.28 and 117.93±1.96) unit/L, alkaline phosphatase (3.60±0.28 and 2.43±0.11) unit/100 ml, levels at the tested doses, respectively after treatment. However, purified Bauhinia variegate leaves and purified flowers treatment noticeably improved the activity of antioxidant limitations: glutathione, superoxide dismutase, and catalase. The hepatoprotective activity can confirmed by histological finding. From these results it can be concluded that the Bauhinia variegate leaves and flowers extracts contain remarkable of flavonoid can be used as reducing oxidative stress.