Antioxidant Silver-based Biogenic Systems Generated from Arctium lappa Leaves

Marcela Elisabeta Barbinta-Patrascu,Aurelia Meghea,Daniela Besliu

doi:10.37358/rc.20.4.8049

Abstract

This work aims at designing through a �green� bottom-up strategy, four types of bio-based systems containing artificial cell membranes (with or without vitamin C) and silver nanoparticles (AgNPs) phyto-generated from aqueous extract of Arctium lappa leaves. The �green� approaches for AgNP synthesis, by using the vast bioreducing potential of plants are low-cost and eco-friendly procedures.Exploiting vegetal wastes as precursors for phyto-metallic nanoparticles has received attention from scientific community in the last years due to its significant economic and environmental impacts. UV-Vis absorption spectra demonstrated the biosynthesis of metallic nanoparticles which exhibited a strong SPR peak located at 437 nm wavelengths. The formation of the biocomposites containing the �green� synthesized AgNPs has been demonstrated by UV-Vis absorption spectroscopy and Dynamic Light Scattering measurements. DLS results highlighted the nanoscale size of the prepared �green� AgNPs. The physical stability of the obtained silver-based systems was evaluated through zeta potential values, based on electrophoretic mobility. The systems loaded with vitamin C proved to be more stable and showed enhanced antioxidant activities (between 72.3% to 88% evaluated by chemiluminescence technique). Our results demonstrated that these developed silver-based biogenic systems generated from Arctium lappa leaves could be used as antioxidant agents in various biomedical applications.

Highlights

This work develops a “green” bottom-up strategy to achieve antioxidant delivery systems based on vitamin C, biomimetic membranes and biogenic nano-silver synthesized from Arctium lappa leaves aqueous extract
The UV-Vis absorption spectrum of AgNPs “green” synthesized from Arctium lappa leaves showed a strong SPR peak located at 437 nm wavelength, occurring after addition of burdock extract (BE) in 1 mM AgNO3 solution, demonstrating the biosynthesis of silver nanoparticles
Artificial cell membranes and phytogenic silver nanoparticles “green” generated from Arctium lappa extract were used to bottom-up design of antioxidant biogenic systems with and without vitamin C

Summary

Introduction

This work develops a “green” bottom-up strategy to achieve antioxidant delivery systems based on vitamin C, biomimetic membranes (liposomes) and biogenic nano-silver synthesized from Arctium lappa leaves aqueous extract. Liposomes are self-assembling vesicles containing one or more lipid bilayers with a unique structure (similar to the structure of biological membranes) conferring them the ability to load both hydrophilic and lipophilic compounds, and some other interesting properties such as: protection of active agents [1, 2], building block for optoelectronic devices [3], and enhanced stability and improved biological properties (such as: antioxidant, antimicrobial and antiproliferative activities) for silver-based composites [4,5,6,7]. „Green” synthesized silver nanoparticles are recognized for their interesting biological activities.

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