Abstract
Heat stress (HS) induces oxidative stress by increasing reactive oxygen species (ROS), and the polyphenol resveratrol (RSV) has been shown to have antioxidant properties by reducing ROS. Hence, we aimed to examine the effects of RSV, HS and their interaction on bovine adipocytes. We generated bovine dedifferentiated adipocyte-derived progeny (DFAT) cells from subcutaneous adipose tissue and examined the effects of RSV (100 µM), heat conditions: isothermal (ISO-37 °C), short heat (SH-41.2 °C for 1 h) and long HS (LH-41.2 °C for 16 h), and their interaction on gene expression in DFAT-cells. In medium of DFAT-cells treated with RSV, malondialdehyde levels were reduced and oxygen-radical absorbance-capacity levels were increased compared to control. Treating DFAT-cells with RSV increased the relative mRNA expression of stress-induced-phosphoprotein-1 (STIP1) and the expression of hormone-sensitive-lipase (LIPE) and perilipin-1 (PLIN1), whereas it reduced the expressions of fatty-acid-synthase (FASN) and of pro-inflammatory chemotactic-C-C-motif-chemokine-ligand-2 (CCL2) also under HS. Moreover, reduced protein abundance of FASN was found in RSV-treated DFAT-cells compared to controls. Molecular docking of RSV with FASN confirmed its possible binding to FASN active site. This work demonstrates that RSV has an antioxidant effect on bovine DFAT cells and may induce adipose lipolysis and reduce lipogenesis also under in vitro HS conditions.
Highlights
Introduction iationsIn dairy cows, environmental heat stress (HS) leads to decreased productive and reproductive functions [1,2]
Environmental HS caused by high ambient temperature and humidity conditions induces NF-E2-related factor2 (NRF2) mediated oxidative stress pathway in adipose tissue of late pregnancy dairy cows by affecting several biomarkers and increasing reactive oxygen species (ROS) along with lipid peroxides [3,4,5]
DFAT cells, we examined the relative expressions of heat shock factor 1 (HSF1), super oxide dismutase 1 (SOD1) and stress induced phosphoprotein 1 (STIP1) genes under ISO, SH and long heat stress (LH) conditions (Supplementary Materials Table S2)
Summary
Environmental heat stress (HS) leads to decreased productive and reproductive functions [1,2]. Environmental HS caused by high ambient temperature and humidity conditions induces NF-E2-related factor (NRF2) mediated oxidative stress pathway in adipose tissue of late pregnancy dairy cows by affecting several biomarkers and increasing reactive oxygen species (ROS) along with lipid peroxides [3,4,5]. The imbalance of ROS can be improved by the complex antioxidant system, maintaining redox balance in cells. In this antioxidant defense system, different types of antioxidant enzymes and non-enzymatic low molecular weight antioxidants play an important role [10]. HS causes interruption in these enzyme expressions and functions and accumulates superoxide anion
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
