Antioxidant response to postprandial lipemia in active and sedentary men

Abstract

This study compared the postprandial lipemia antioxidant status in healthy males, active and sedentary. Nine active and 8 sedentary males (18–38 yrs old) were recruited based on health (ACSM Risk Criteria) and fitness (VO2 peak on a cycle ergometer, 64.1 and 39.4 mL/kg/min, respectively). Plasma antioxidant status was investigated after 36 h of inactivity and 12 h of fasting (0 h) and at 3 h and 6 h after consumption of a high fat dairy based shake. Plasma levels of thiobarbituric acid reactive substances (TBARS) and antioxidant enzyme activity of glutathione reductase (GSR), glutathioneperoxidase (GPx), glutathione transferase (GST), and superoxide dismutase (SOD) were determined. TBARS levels did not change over time in either group, but on average the active group was 21% higher than sedentary. At 0 h, GSR was 19% lower in the active group than sedentary. In the active group, GSR decreased by 17% from 3 h to 6 h. GPx was higher at 0 h and 3 h for the active group but not at 6 h. GST and SOD were similar between groups. Being physically active may be associated with elevated activity of TBARS and GPx, decreased activity of GSR, while other antioxidant activity remains unchanged. Postprandial lipemia caused by a high fat dairy meal did not increase TBARS, and had little effect on antioxidant enzymes. MDFR Grant, Univ. at Buffalo