Abstract
The present study established a protocol for suspension cell culture of Stevia rebaudiana and demonstrates the antioxidant enzymes activity during various developing phase of it. Higher concentration of Auxin and Cytokines (3.0 mg L -1 BAP and 4.0 mg L -1 NAA) with Ascorbic acid (1 mg L -1 ) considered as highly suitable growth regulator combination for growth and development of Stevia rebaudiana suspension culture. Maximum concentration of stevioside (~70 mg G -1 of dry weight of tissue) was obtained on 14 th day (exponential phase) which got reduced on 28 th day (apoptotic phase) upto ~21 mg G -1 of dry weight of tissue. The amount of MDA reduced initially upto stationary growth phase which showed the adaptation of suspension cells in the culture medium and culture environment. Reduction of Chlorophyll showed the enhancing phenolic content with progressive culture period, while enhanced amount of proline was indicating the generation of defense mechanism with depletion of nutrient (with increased culture duration). The amount of SOD got enhanced with elevating the concentration of H 2 O 2 as well. The concentration of CAT enhanced upto stationary growth phase of suspension cell while APX concentration showed continuous inhibition up to apoptotic phase from exponential phase.
Highlights
Stevia rebaudiana is a budding plant of the Asteraceae family in the field of commercial sweetener market (Sreedhar et al, 2008)
The cell suspension cultures are the technique for rapid and uniform production of single cells which are helpful for the production of high quantity of secondary metabolites (Davies and Deroles, 2014)
The present study provides a platform for long term culture of suspension cells of S. rebaudiana
Summary
Stevia rebaudiana is a budding plant of the Asteraceae family in the field of commercial sweetener market (Sreedhar et al, 2008). Various biotechnological methods are applied for enhanced production of secondary metabolites These methods include organ culture of plant, addition of precursor, elicitation of in vitro products, hairy root cultures, genetic manipulation and Horizon e-Publishing Group bioreactor scaling up production. Among all these techniques, the tissue culture techniques are simpler and have broader criteria. On the other hand plant cell contains a wide variety of defensive systems These systems (enzymatic and non enzymatic antioxidant system) react with active mode of oxygen and maintain them at low level, so that cells can grow at abnormal culture condition as well (Allen, 1995). The present study provides a platform for long term culture of suspension cells of S. rebaudiana
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
