Antioxidant protection enzyme activity in the blood serum and large intestinal mucosa of rats with prolonged gastric hypochlorhydria and given multiprobiotics

S V Pylypenko,A A Koval

doi:10.15421/021965

Abstract

The activity of antioxidant protection enzymes in the blood serum and colon mucosa in rats was studied under the conditions of 28-days administration of omeprazole on its own and omeprazole together with multiprobiotics "Symbiter" and "Apibact". Physiological and biochemical study methods were applied. It was found that after omeprazole administration, the activity of superoxide dismutase in the blood serum decreased, and the activity of catalase increased compared to the control. With the co-administration of omeprazole and multiprobiotics, the activity of superoxide dismutase increased compared to the group of rats that received omeprazole only during the same time, but remained less compared to the control group. The content of reduced glutathione in the blood serum of rats after administration of omeprazole decreased, the activity of glutathione peroxidase and glutathione transferase increased, and the activity of glutathione reductase decreased compared to the control. With co-administration of omeprazole and multiprobiotics, the serum RG content was at the control level, the activity of glutathione reductase exceeded the control values. The activity of glutathione reductase decreased compared to the group receiving omeprazole only. The activity of glutathione reductase increased and did not differ from the control values. In the colon mucosa, superoxide dismutase and catalase activity decreased compared to control. With the combined administration of omeprazole and multiprobiotics, superoxide dismutase and catalase activity increased and even exceeded the control values. With the administration of omeprazole, the reduced glutathione content in the colon mucosa was lower than that in the control. The activity of glutathione peroxidase increased and glutathione transferase and activity of glutathione reductase decreased compared to the control. With co-administration of omeprazole and multiprobiotics to rats, the reduced glutathione content increased compared to the group of rats administered omeprazole only, and even exceeded that in the control.

Highlights

An important problem for research remains the functioning of the digestive system in the conditions of prolonged gastric hypochlorhydria, which leads to hypergastrinemia, which in its turn is known to be a significant factor in the growth and development of tumours in the gastrointestinal tract (Burkitt еt al., 2009)
The results suggest that after prolonged inhibition of hydrochloric acid (HCl) secretion in the stomach, the glutathione antioxidant defense link is depleted in the colon mucosa, as a result of the over-activation of free radical reactions and LPO, and the cells cannot resist the oxidative stress against the background of inflammatory process induced by hypergastrinemia and dysbiosis
Prolonged gastric juice hypochlorhydria led to changes in the functioning and depletion of antioxidant protection enzymes: compared to the control, the blood serum superoxide dismutase (SOD) activity decreased by 54.5% (P < 0.05) and the catalase activity increased by 211.5% (P < 0.05)

Summary

Introduction

An important problem for research remains the functioning of the digestive system in the conditions of prolonged gastric hypochlorhydria, which leads to hypergastrinemia, which in its turn is known to be a significant factor in the growth and development of tumours in the gastrointestinal tract (Burkitt еt al., 2009). Most frequently carcinoid tumours of the stomach develop in patients in whom diseases are associated with hypergastrinemia (Stepanov еt al., 2000). These are diseases such as chronic atrophic gastritis (Sipponen et al, 2002), Zolinger-Ellison syndrome with multiple endocrine neoplasia type 1 (Zhong et al, 2005), pernicious anemia (Kedika, 2009) and conditions after surgery for vagotomy (Waldum et al, 2005). It is known that decrease in gastric juice acidity for any reason can lead to excessive bacterial growth in the oral cavity, the stomach (Habig et al, 1974; Watson et al, 2002; Willams & McColl, 2006, 2018; Pylypenko et al, 2018) and in the small and large intestine (Habig et al, 1974; Osefo et al, 2009)

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