Antioxidant Properties of Soybean (Glycine max L.) Extract and Isoflavone

Abstract

Free radicals caused oxidative stress in the body, which leads to various chronic and degenerative diseases. The negative effects of free radicals can be neutralized by natural antioxidants. Soybean (Glycine max L.) extract contains isoflavones that have several biological activities, including antioxidants. Soybean extract (SE) antioxidant activity was evaluated compared with isoflavones (ISO). The SE was extracted in aquademineral solvent and additional lactose. SE and ISO were subject to various antioxidant activity assay such as 2,2-diphenyl-1-pycrylhydrazyl (DPPH), 2,2’-azinobis-3-ethylbenzo-thiazoline-6-sulfonicacid (ABTS), H 2 O 2 , NO scavenging activities and ferric reducing antioxidant power (FRAP) assay, following standard procedure. As a results, the IC 50 of DPPH, ABTS, H 2 O 2 , NO, of SE were 246.51; 35.96; 289.41; 39.74 µg/mL respectively. While the IC 50 value of isoflavones were 71.37; 23.57; 259.50; 11.59 µg/mL respectively. Furthermore, at the higher concentration (50 µg/mL) of SE and ISO’s FRAP activity were 196.89 and 177.78 μM Fe(II)/μg. Even though the antioxidant activity of SE is lower than isoflavones, SE still has antioxidant potential. Thus, it can be used for supplement candidate.