Abstract
Six compounds hispidulin-7-glucuronide ( 1), β-sitosterol ( 2), conferyl aldehyde ( 3), 2′-hydroxyl-5′-methyoxybiochanin A ( 4), and hispidulin-7- O- d-glycoside ( 5), 6-methoxyluteolin-7-glycoside ( 6) were isolated from Salvia plebeia and identified by UV, IR, Mass, 1H and 13C NMR spectra. Their antioxidant activities were investigated individually and compared with α-tocopherol by the oxidative stability instrument (OSI) at 40 °C. The oils extracted from Styela clava and Ciona intestinalis Linnaeus with supercritical fluid (SCF) CO 2 and ethyl acetate, respectively, were used as substance oils, which were rich in polyunsaturated fatty acids (PUFA), especially in the long chain PUFA icosapentaenoic acid (EPA, 20:5 n − 3) and docosahexaenoic acid (DHA, 22:6 n − 3). β-Sitosterol, 2′-hydroxyl-5′-methyoxybiochanin A and 6-methoxyluteolin-7-glycoside exhibited strong antioxidant activities, which increased markedly with increasing concentration, and even stronger than α-tocopherol. The results indicate that the components extracted from S. plebeia should preserve the quality of ascidian oil from ascidian oxidative deterioration.
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