Abstract
The antioxidant profiles of 19 lemon balm leaf samples collected in Hungary and extracted with methanol were investigated by videodensitometric high-performance thin-layer chromatography–1,1-diphenyl-2-picrylhydrazyl radical scavenging (HPTLC–DPPH•) assay detection versus the respective microtiter plate assay. Appropriate separation of the antioxidants was achieved on the HPTLC cyano plate with acetonitrile—water—acetic acid 20:30:1, V/V/V, as mobile phase. All samples provided a similar antioxidant profile with rosmarinic acid as the main active component identified by HPTLC–high-resolution mass spectrometry (HRMS). The total antioxidant effects of the samples obtained by the two different techniques showed a good correlation. The influence of the collection time and area as well as of the phenophase of the plant on the activity was evident. For samples from the same population over a season, the total antioxidant effect increased until the flowering (mid-July), and after a slight drop during flowering (in August), it returned to a high level again (in September).
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