Abstract
Squid ink has secondary metabolites to avoid enemy attacks. Secondary metabolite compounds have been able to determine the presence of antioxidant activity. Maceration is a way to get extracts and secondary metabolites in a sample. This study aimed to identify secondary metabolites and determine antioxidant activity in L. duvauceli ink extract. The method in this research was descriptive. The treatments used were solvents with different polarity levels, n-hexane, ethyl acetate, and ethanol. The parameters carried out in this study were yield, qualitative and quantitative secondary metabolites compound testing, and antioxidant activity testing. The results showed that n-hexane extract showed the presence of saponin compounds, ethyl acetate extract contained steroid compounds, and ethanol extract contained alkaloids and phenolic compounds. Saponin content in n-hexane extract was 0.23%. The levels of alkaloids and phenolics in the ethanol extract were 0.02% and 0.72%, respectively. The results show that L. duvauceli ink has antioxidant activity obtained from the IC50 value, n-hexane extract was 128.35 ppm, ethyl acetate extract was 78.81 ppm, and ethanol extract was 75.68 ppm.
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