Antioxidant potential of Quartzetin and Rosemary extract as components of Nanometric apatite biopolymer materials for osteoplasty

Abstract

Significant oxidative stress is induced in the bone implant surrounding tissues is a well-known problem. It's expected that the introduction of antioxidant substances (AOS) – Quercetin (Qu) and Rosemary Extract (RE) into apatite-biopolymer composites for osteoplasty as potential drug carriers will affect the antioxidant activity (AOA) and promote the natural process of the damaged bone tissues regeneration due to the active absorption of reactive oxygen species (ROS). In the present work was synthesized hydroxyapatite(HA)-Alginate(Alg) matrix, doped with micro-(ZnOMPs), nano-(ZnONPs) particles, Zn2+ ions and loaded with Qu and RE. The dependence of AOA on the method of Qu and RE loading, as well as the influence of the inorganic component (HA, ZnONPs/MPs, Zn2+) and exposure time in methanol, phosphate buffer (PBS), was analyzed. Hydrophilic RE and hydrophobic Qu are evenly distributed in the matrix volume under sonication, which can slow down their diffusion, contributing to prolonged activity. The highest AOA after 54h showed ZnONPs containing samples with Qu loaded both during synthesis (76.93 %) and saturation (93.97 %) and RE-saturated samples with ZnONPs and Zn2+ (88.53 and 75.33 %, respectively). The adsorption and retention of AOS by matrix components also take place.